LIFE Study Ancillary Tracking Summary Report

Approved and Conditionally Approved Ancillary Studies

Reference # Title Principle
Investigator
Contact Information Status* Status Date Completed/In Processs Study Type Summary of Ancillary Study
AS10-02  A neuroimaging study to measure the association of baseline cerebral blood flow, structural brain characteristics and neuronal activation with the 2-year response to physical activity intervention in the LIFE –main participants.   Rosano, Caterina  Phone:  
Email: rosanoc@edc.pitt.edu
Ancillary Study Approved  04/05/2012    This proposal supplements resources already in place to test the hypothesis that baseline measures of brain integrity may be predictive of positive outcomes in response in physical activity. Additionally, we propose that PA changes are associated with differences in ultrastructure of hippocampal subregions and vascularization. We also propose to explore potential mechanisms underlying these associations, including levels of brain derived neurotrophic factors at the 2-year follow-up visit . Neuroimaging measures of brain integrity will include cutting–edge markers of cerebral blood flow and structural characteristics using a 7 Tesla magnet. 
AS12-10  Analysis of Coding Variants Associated with Age-Related Phenotypes  Bowden, Donald  Phone: (336) 713-7507  
Email: dbowden@wfubmc.edu
Ancillary Study Approved  03/22/2013    The aim of the proposed project is to examine common and uncommon coding variants for association with a wide range of biomedical measures characteristic of aging. Using DNA from participants in the LIFE-P study, coding variants associated with agerelated phenotypes will be genotyped using the Sequenom® MassArray Genotyping platform and analyzed for associations with particular age-related phenotypes using data previously collected in the LIFE-P study. A variance components analysis will be performed using the Sequential Oligogenic Linkage Analysis Routines (SOLAR) software package (Texas Biomedical Research Institute), which robustly controls for bias introduced by alleles that are identical by descent due to the family-based structure of the DHS cohort 
AS13-03  Autonomic function and gait variability in older adults at risk for mobility disability  Marsh, Anthony  Phone: (336) 758-4643  
Email: marshap@wfu.edu
Ancillary Study Approved  09/13/2013    The aim of this ancillary study is to describe the degree of dysregulation in, and the relationship between, both autonomic function and gait using state-of-the-art non-linear dynamic analyses of HRV and step-to-step intervals of gait during both normal- and fast-paced walking. We will then examine whether dysfunction in HR and gait variability, either individually or in combination, is related to mobility. In addition, we will examine whether dysregulation in either HR or gait variability assessed during walking is moderated by confidence in balance, demographic characteristics (age, sex), and disease burden.  
AS12-11  Cerebral metabolic, vascular and functional neuroimaging, NIH Toolbox cognitive measures and physical activity as predictors of age-associated cognitive dysfunction.  Cohen, Ron  Phone: (352) 294-5800  
Email: roncohen@ufl.edu
Ancillary Study Approved  12/12/2012    Using previously established neuroimaging methods (T1 MPRAGE, FLAIR, DTI, MRS, ASL, resting BOLD), we propose to collect in vivo MRI measures from participants in the University of Florida cohort during a one hour multimodal scanning session. Data from this assessment will provide information regarding brain structural, metabolic/physiological and functional disturbances. Neurocognitive function will be examined relative to each neuroimaging modality using statistical modeling methods to determine the extent to which they predicts performance in specific cognitive domains (learning-memory, attention-executive, motor-processing speed). Coupling MRS, ASL and FMRI measures will enable us to examine how cerebral metabolic, vascular and functional changes are inter-related. Serum blood samples will also be collected and stored for analysis at a later date. Potential analyses include previously investigated serum biomarkers, such as IL-6, MCP-1, TNF- a, and BDNF. 
AS13-07  CKD progression in older adults: the impact of geriatric conditions and physical activity  Liu, Christine  Phone:  
Email: christine.liu@bmc.org
Ancillary Study Approved  02/05/2014    Specific Aim 1: To determine if geriatric conditions are associated with increased risk of CKD progression in older adults with CKD. Hypothesis: Those who have exhaustion, weight loss, dizziness, depression, cognitive impairment, poor physical function, frailty, urinary problems, and/or falls at baseline will be more likely to develop CKD progression at 12 and 24 months. Specific Aim 2: To determine if physical activity can prevent CKD progression in older adults with CKD. Hypothesis: Those who are randomized to the physical activity (PA) group will be less likely to develop CKD progression at 12 and 24 months, compared to those randomized to the successful aging (SA) group. Exploratory Aim: To determine if inflammation is reduced after a program of physical activity in older adults with CKD. Hypothesis: In older adults with CKD, the inflammatory markers of interleukin-6 and C reactive protein will be reduced at 12 and 24 months in those randomized to the physical activity intervention, compared to those in successful aging. 
AS09-10  Complex Mobility and Executive Functioning  Katula, Jeffrey   Phone: (336) 758-3612  
Email: katulaj@wfu.edu
Ancillary Study Approved  02/22/2010    Participants will be required to complete 3 clinic visits in addition to the LIFE Study required visits (baseline, 12 mo, 24 mo). Each visit will take approximately 30 minutes. During each visit participants will complete 1 computerized cognitive assessment (10 min), the Walking Decision Making Task (8 min), and a complex walking task (from the INCHIANTI toolbox; 5 min). 
AS16-03  Diabetes-Related Mechanisms Underlying Cognitive Changes with Exercise and Aging  Brinkley, Tina  Phone: (336) 713-8534  
Email: tbrinkle@wakehealth.edu
Ancillary Study Approved  05/25/2016  Main Trial
 
The proposed ancillary study will examine potential diabetes-related mechanisms that may influence changes in cognition in response to the Physical Activity intervention. Specifically, the study will use a comprehensive phenotyping approach to determine the role of the AGE/RAGE pathway, which has been shown to affect neurodegenerative processes through mechanisms involving glycation, oxidative stress, and inflammation. Additionally, the study will determine the relationship between AGE/RAGE-related biomarkers and incident mild cognitive impairment (MCI) and dementia.  
AS16-04  Dopamine-related genes, physical activity adherence, and cognitive outcomes  Rosso, Andrea  Phone: (412) 383-1066  
Email: alr143@pitt.edu
Ancillary Study Approved  06/09/2016  Main Trial
 
We propose to add genotyping for single nucleotide polymorphisms (SNPs) in 8 relevant genes (COMT, DAT, DRD1, DRD2, DRD3, DRD4, DRD5, and BDNF) to the full sample of LIFE participants. The results of this proposal will provide evidence for the role of dopamine-related pathways in physical activity adherence in older adults. These proof of concept results will be used to pursue funding for novel intervention strategies harnessing the dopaminergic system to improve physical activity adherence in older adults. Baseline, cross-sectional associations between polymorphisms of the 8 genes with the composite measure of executive function and with gait speed will be tested for the full LIFE sample using regression models. These analyses will be adjusted for field center and demographic and health variables associated with cognitive and physical function. Gene-gene interactions will be tested. 
AS12-06  Effects of a one-year physical activity program (LIFE-P) on serum C-terminal Agrin Fragment (CAF) concentrations among older adults at risk for physical disability   Buford, Thomas  Phone: (352) 294-5800  
Email: twbuford@uab.edu
Ancillary Study Approved  11/13/2012    Evaluate the effects of the LIFE-P interventions on circulating concentrations of a C-terminal Agrin Fragment (CAF) 
AS05-01  Effects of exercise training on prevalence of metabolic syndrome in the elderly  Nicklas, Barbara  Phone: (336) 713-8037  
Email: bnicklas@wfubmc.edu
Ancillary Study Approved  05/25/2005    This ancillary study will utilize existing blood specimens at each assessment time point from all LIFE participants (regardless of age, gender or intervention assignment) who provided informed consent for phlebotomy. There is no extra time or effort required by participants. The ancillary study will not significantly impact the main LIFE study. We propose to generate biological data from the blood specimens, specifically, lipoprotein lipid, glucose and insulin concentrations, as well as inflammatory markers (CRP, IL-6, TNF?, sTNFR1 and sTNFR2). Analysis of the metabolic factors will allow us to determine the number of individuals at each assessment time point who have metabolic syndrome to address our primary hypothesis. Analysis of the inflammatory markers will allow us to assess whether improvements in metabolic syndrome components are related to reductions in inflammation (secondary hypothesis).  
AS16-01  IL-6 level and walking speed in the LIFE Study: Participation in a Meta-Analysis of the Association of Inflammation with Mobility and Mobility Disability.  Tracy, Russell  Phone:  
Email: Russell.Tracy@uvm.edu
Ancillary Study Approved  04/21/2016    Measurement of IL-6 in LIFE-M samples to help inform study design for ENRGISE main study Existing serum samples (baseline and 12 month follow-up) from all participants from the LIFE study will be analyzed for IL-6 level. IL-6 will be measured by the most commonly used method, the HS R&D Systems immunoassay. The LCBR at the University of Vermont has extensive experience with this assay, and was the laboratory that measured IL-6 in Health-ABC and TRAIN. 
AS12-02  Impact of moderate physical activity on the longitudinal trajectory of cardiac specific biomarkers of stress and injury: support for modifying early heart failure phenotypes  deFilippi, Christopher  Phone: (410) 328-7204  
Email: cdefilip@medicine.umaryland.edu
Ancillary Study Approved  09/25/2012    We propose to measure NT-proBNP,cTnT and cTnI in the Lifestyle Interventions and Independence for Elders (LIFE) pilot study and identifying if the frequency and extent of a rise in concentrations of any or all biomarkers are attenuated in those undergoing an active intervention of physical activity. 
AS09-02  LIFE-KIDNEY Ancillary Study  Shlipak, Michael  Phone: (415) 221-4810 x3381 
Email: michael.shlipak@ucsf.edu
Ancillary Study Approved  04/11/2011    The LIFE-KIDNEY ancillary study would extend the breadth of the trial by adding the kidney endpoints of kidney function (estimated glomerular filtration rate [GFR] from cystatin C and creatinine) and kidney damage (albuminuria). Both eGFR using cystatin C and albuminuria are very powerful prognostic markers in elderly persons, but no clinical trial in the elderly has ever attempted to delay the onset and progression of kidney disease. In the Cardiovascular Health Study (CHS), an observational study, physical inactivity was the strongest of all risk factors for more rapid decline in kidney function. Therefore, we believe that kidney endpoints would greatly enrich LIFE. 
AS11-02  Modification of HDL Function by Physical Training in the Elderly  Tang, W. H. Wilson  Phone:  
Email: tangw@ccf.org
Ancillary Study Approved  05/20/2014    The proposed studies will provide a comprehensive evaluation of the role that multiple pathways have in modifying HDL function, the development of CAD, and its major adverse complications such as coronary artery disease, heart failure, and death, as well as the relation between physical activity and these novel cardiometabolic risk factors by leveraging an ongoing physical activity intervention trial in the elderly population. Successful completion of the proposed studies will provide detailed mechanistic and clinical insights into the potential for novel cardiometabolic risks associated with modified HDL function to serve as a therapeutic target and/or diagnostic tools for cardiovascular diseases in at-risk patients. 
AS12-13  mtDNA modifiers of the effect of exercise on cardiopulmonary and walking function in elders  Manini, Todd  Phone: (352) 294-5800  
Email: tmanini@ufl.edu
Ancillary Study Approved  04/19/2017    Our central hypothesis is that mtDNA sequence variation explains a portion of the heterogeneity in cardiopulmonary and walking speed responses to regular PA MtDNA sequence variants will be quantified both individually and jointly as detailed below. In-silico methods will then be employed to examine mtDNA nucleotide conservation and the impact of coding substitutions on amino acid protein sequences. In cases where a strong individual variant association is identified we will assess functional impact via in-silico predictions and a unique source of information for mtDNA gene/variant function  
AS12-01  One-carbon metabolism and its relationship to physical and cognitive performance  Quinlivan, Eoin  Phone: (352) 273-5721  
Email: epq@ufl.edu
Ancillary Study Approved  07/18/2012    This study will explore the relationship between one-carbon metabolism and physical (SPPB, 400 m walk, grip strength) and cognitive (DSST, Modified Stoop test, 3MSE, RAVLT) function measures, and the effect exercise has on this relationship Existing citrated plasma samples (baseline and 6 month follow-up samples) from participants in the LIFE-P study will be analyzed for one-carbon metabolite levels. One-carbon metabolite levels will be measured in citrated plasma samples using a LC-MS/MS assay developed by the University of Florida Clinical and Translational Science Institute’s Biomedical Mass Spectrometry Laboratory, and by established laboratory methods. 
AS13-04  Peripheral metabolites associated with cognitive performance in participants of the Lifestyle Interventions and Independence for Elders Pilot study   Herzog, Raimund  Phone:  
Email: raimund.herzog@yale.edu
Ancillary Study Approved  07/10/2013    This study will explore the relationship between circulating peripheral plasma metabolites and cognitive function (as measured via a cognitive testing battery that includes the Modified Mini-Mental State Exam (3MSE); the Rey Auditory Verbal Learning Test (RAVLT) a test of working memory; the Digit Symbol Substitution Test (DSST), which tests mental speed and working memory and the Modified Stroop Test, which tests executive function). Based on compelling evidence from the literature, our focus will especially be on short chain acyl-carnitine species, which if deficient, have been associated with both physical and mental fatigue and decline We will utilize existing plasma samples (baseline and 12 month follow-up samples) from participants in the LIFE-P study to generate information about their circulating acylcarnitine, amino acid, hexoses, phospho- and sphingolipid and biogenic amines levels by mass spectrometry conducted at the Keck mass spectrometry core at Yale School of Medicine 
AS11-07  Physical Activity and Depressive Symptoms in LIFE-P: Effects of Genetic Polymorphisms and Symptom Dimensions of Depression  Dotson, Vonetta  Phone:  
Email: vonetta@phhp.ufl.edu
Ancillary Study Approved  02/14/2012    the goal of the proposed research is to help clarify the relationship between physical activity and depressive symptoms by examining 1) the effect of exercise on particular symptom dimensions of depression, 2) whether certain genetic markers moderate the effect of exercise on depressive symptoms, and 3) the relationships between depressive symptoms, exercise and cognitive functioning. 
AS14-03  Physical activity effects on blood biomarkers of neurogenesis, angiogenesis, and inflammation and its impact on mobility and cognition  Sarles, Beth   Phone: (412) 383-1294  
Email: cesst52@pitt.edu
Ancillary Study Approved  03/13/2015  Main Trial
 
 
AS11-08  Quantifying Physical Activity in the Physical Activity Intervention Using Accelerometry  Rejeski, Jack  Phone: (336) 758-5837  
Email: rejeski@wfu.edu
Ancillary Study Approved  04/05/2012    Our interest is to evaluate the intensity and duration of walking activity, and to determine the number of bouts during a typical walking session (the number of breaks). These data will (a) help us to better understand how to interpret accelerometry data that is collected on similar populations in free-living environments, (b) provide a quantitative description of actual walking behavior that is being performed by participants in the LIFE study, and (c) enable us to determine whether there are reliable predictors of physical activity behavior in this context.  
AS14-01  Role of Genetic Variation in the Impact of Physical Activity on Depressive Symptoms and Cognitive Functioning  Dotson, Vonetta  Phone:  
Email: vonetta@phhp.ufl.edu
Ancillary Study Approved  09/26/2014     
AS16-02  Telomere Length in Older Adults with Functional Limitations following a 12 Month Exercise or Education Intervention   Sibille, Kimberly  Phone: (352) 294-5846  
Email: ksibille@ufl.edu
Ancillary Study Approved  08/17/2016  Main Trial
 
 
AS10-01  The Effect of the Life Interventions on Global Gene Expression   Chupp, Geoffrey  Phone:  
Email: geoffrey.chupp@yale.edu
Ancillary Study Approved  09/18/2010     
AS10-01  The Effect of the Life Interventions on Global Gene Expression   Chupp, Geoffrey  Phone:  
Email: geoffrey.chupp@yale.edu
Ancillary Study Approved  09/18/2010    This study will elucidate how the LIFE intervention affects global gene expression in the blood and will help to identify gene profiles of responsiveness to the LIFE intervention 
AS13-01  The Effects of Exercise on Apelin  Dray, Cedric  Phone:  
Email: cedric.dray@inserm.f r
Ancillary Study Approved  03/22/2013    This study will explore the effects of physical activity on plasma apelin as well as the relationship between plasma apelin and physical performance measures (SPPB, 400 m walk), grip strength, IL-6, CRP, and DEXA measures of lean appendicular mass 300 existing plasma/serum samples (baseline and 6 and 12 month follow-up samples from 100 participants, 50 randomly selected from the PA group and 50 randomly selected from the SA group) from the LIFE-P study will be analyzed for apelin level. Apelin levels will be measured in either serum or plasma samples using a commercially available enzyme-linked immunosorbent assay.  
AS11-06  The Effects of Exercise on Procollagen-3 N-Terminal Peptide (P3NP)  Pahor, Marco  Phone: (352) 294-5800  
Email: mpahor@ufl.edu
Ancillary Study Approved  01/18/2012    This study will explore the effects of exercise on P3NP as well as the relationship between P3NP and physical performance measures (SPPB, 400 m walk), grip strength, IL-6, CRP, and DEXA measures of lean appendicular mass. The proposed ancillary study will utilize existing serum or plasma samples collected as a part of the LIFE Pilot study. Existing citrated plasma samples (baseline and 6 month follow-up samples from all participants from the LIFE-P study will be analyzed for P3NP level. P3NP levels will be measured in citrated plasma samples using a commercially available enzyme-linked immunosorbent assay.  
AS12-12  The impact of chronic kidney disease on the effectiveness of physical activity in older adults  Liu, Christine  Phone:  
Email: christine.liu@bmc.org
Ancillary Study Approved  11/28/2012    The data generated by this analysis will provide information on whether a physical activity to improve physical function is a feasible and effective intervention for older adults with CKD. We would like to analyze stored blood samples for cystatin C. We would likely have the samples shipped from Wake Forest and have them analyzed at Tufts 
AS11-03  The LIFE Study: Mitochondrial Function and Exercise: Relationship with Function and Fatigue.   Goodpaster, Bret  Phone: (412) 692-2437  
Email: Bret.Goodpaster@FLHosp.org
Ancillary Study Approved  06/09/2011    In this proposed pilot ancillary study, mitochondrial function will be measured directly and in-vivo via the novel and non-invasive method of 31P magnetic resonance spectroscopy (MRS). This new technology involves little participant burden, whereas the traditional method of muscle needle biopsy is highly invasive, induces considerable participant burden and must be performed by a physician. 

 

 

Withdrawn Ancillary Studies
Previously approved studies that have been withdrawn are listed below. It is recommended that new proposers of ancillary studies contact the principal investigators of these withdrawn proposals, who may have some helpful insight for proposers of new ancillary studies.
Reference # Title Principle
Investigator
Contact Information Status* Status Date Completed/In Processs Study Type Summary of Ancillary Study
AS13-06  ACE pharmacogenetics and behavioral medicine to prevent age-related disability   Buford, Thomas  Phone: (352) 294-5800  
Email: twbuford@uab.edu
Withdrawn  11/21/2016    1) Determine if ACE I/D genotype influences the effect of ACEi+EX on changes in physical function among older adults. Based on our preliminary work, we hypothesize that changes in physical function in response to ACEi+EX will be poorest among persons with the II genotype. 2) Identify additional SNPs in ACE-related genes which may influence the effect of ACEi+EX. We propose to test the influence of other putative functional SNPs on changes in physical function in response to ACEi+EX. 3) Evaluate how the targeted genotypes influence changes in physical function in response to other antihypertensive medications (e.g. ARBs, diuretics) combined with exercise. These data will serve as a negative control to test that the hypothesized pharmacogenetic effects are specific to ACE inhibitor use. 4) Assess the influence of ACE-related genotypes on physical function in response to ACE inhibitor use and health education. These data will serve as a negative control to test our hypothesis that physical exercise is required to observe the hypothesized pharmacogenetic effects.  
  Autonomic Function and Gait Variability in Older Adults At Risk for Mobility Disability  Marsh, Anthony  Phone: (336) 758-4643  
Email: marshap@wfu.edu
Withdrawn  11/07/2013     
AS12-09  Cerebral metabolic, vascular and functional neuroimaging and physical activity as predictors of age-associated cognitive dysfunction  Cohen, Ron  Phone: (352) 294-5800  
Email: roncohen@ufl.edu
Withdrawn  06/12/2013     
AS12-03  Changes in Pain Report, Functioning, and Inflammatory Measures in Older Adults over a 12 Month Period: Exploring Associations with Telomere Length  Sibille, Kimberly  Phone: (352) 294-5846  
Email: ksibille@ufl.edu
Withdrawn  04/01/2016    Differences in clinical pain report, physical performance (SPPB, 400 m walk), quality of life, and inflammatory measures from baseline and at the 12 month time point and examine associations with telomere length. 
AS12-07  Effects of an Exercise Intervention on Vascular and Endothelial Function in the Elderly  Anton, Stephen  Phone: (352) 294-5800  
Email: santon@ufl.edu
Withdrawn  01/06/2015    To test the dose-response effects of an exercise intervention on emerging CVD risk factors 
AS06-01  Environmental Influences on Physical Activity in Older Adults at Heightened Risk for Disability  King, Abby  Phone: (650) 725-5394  
Email: king@stanford.edu
Withdrawn  05/10/2013     
AS03-04  Exercise and changes in Heart Rate Variability (HRV), in sedentary elderly men and women  Church, Timothy  Phone: (225) 763-2632  
Email: tim.church@pbrc.edu
Withdrawn  05/09/2013     
  Exploring the association of low levels of physical activity with disability in older adults  White, Dan  Phone:  
Email: dkw@udel.edu
Withdrawn  11/07/2013     
AS12-14  Genetic associations with exercise-derived improvements in physical function among pre-disabled older adults: LIFE Genetics.  Buford, Thomas  Phone: (352) 294-5800  
Email: twbuford@uab.edu
Withdrawn  11/21/2016    we aim to conduct a genome-wide association study (GWAS) using DNA samples collected from participants in LIFE-M and LIFE-P 
  Is the age-associated decline in cardiorespiratory capacity attenuable/ reversible through exercise training (Fitness Testing)  Church, Timothy  Phone: (225) 763-2632  
Email: tim.church@pbrc.edu
Withdrawn  11/07/2013     
AS11-04  LIFE Study: The effect of physical activity on sleep-wake disturbances  Fragoso, Carlos  Phone: (203) 932-5711  
Email: carlos.fragoso@yale.edu
Withdrawn  01/06/2015    The ancillary sleep testing that we propose to initiate at the 30 month follow-up visit (F30) is conducted only after the main study measures are completed. Specifically, after completing the F30 evaluation, LIFE staff will explain the ancillary sleep study to eligible participants. If enrolled, participants are consented and instructed on the sleep testing protocol. The actual sleep testing is then self-administered at the participant’s home, over 10-days. This includes an evaluation of sleep-wake disorders, namely circadian rhythm disorders, sleep apnea, and movement disorders 
AS10-03  Machine learning of brain shape and composition for clinical prognosis in neural disorders  Laurienti, Paul  Phone:  
Email: plaurien@wfubmc.edu
Withdrawn  11/07/2013     
AS13-05  Metabolomic Predictors of Lean Mass and Physical Function in Functionally- Limited Older Adults  Fielding, Roger  Phone: (617) 556-3016  
Email: roger.fielding@tufts.edu
Withdrawn  11/13/2015    Our overarching hypothesis is that specific serum metabolomic signatures can be identified that are: a) associated with lean mass and physical functioning, b) predictive of changes in lean mass and physical functioning in response to a physical activity intervention, and c) are altered in response to a physical activity intervention resulting from the underlying metabolic changes that result 
AS09-01  Mitochondrial DNA and p53 damage in blood cells of the elderly: Correlation with physical activity (PA)  Sharp, J.G.  Phone: (402) 559-4390  
Email: jsharp@unmc.edu
Withdrawn  05/10/2013     
AS09-07  Muscle quality in the LIFE cohort: Impact of physical activity on changes in muscle strength, power, and composition in community-dwelling older adults at risk for mobility disability.  Goodpaster, Bret  Phone: (412) 692-2437  
Email: Bret.Goodpaster@FLHosp.org
Withdrawn  11/07/2013     
AS09-11  NITRIC OXIDE Ancillary Study  Kim-Shapiro, Daniel  Phone: (336) 758-4993  
Email: shapiro@wfu.edu
Withdrawn  11/07/2013     
AS09-05  Non-exercise activity and risk for major mobility disability among older adults  Manini, Todd  Phone: (352) 294-5800  
Email: tmanini@ufl.edu
Withdrawn  11/07/2013     
AS13-02  Novel biomarkers for response of muscle to exercise in older adults  McLean, Robert  Phone: (617) 971-5376  
Email: rmclean@hsl.harvard.edu
Withdrawn  12/05/2016    The objective of this R01 proposal is to determine circulating biomarkers that are modulated by long-term exercise in older adults, and how they are associated with changes in muscle function. Our central hypothesis is that exercise decreases biomarkers of inflammatory cytokines and TGF-ß muscle atrophy signaling, and increases biomarkers of IGF-1 muscle hypertrophy signaling, and that these changes are associated with improvement in muscle function. 
AS05-02  Novel pathways of the disabling process and physical exercise  Cesari, Matteo  Phone:  
Email: macesari@gmail.com
Withdrawn  11/07/2013     
  One-carbon metabolism and its relationship to physical and cognitive performance.  Quinlivan, Eoin  Phone: (352) 273-5721  
Email: epq@ufl.edu
Withdrawn  11/07/2013     
AS11-01  Physical activity and cardiovascular disease risk in older adults.   Manini, Todd  Phone: (352) 294-5800  
Email: tmanini@ufl.edu
Withdrawn  11/07/2013     
AS09-03  Physiological brain changes with increases in moderate physical activity among previously sedentary older adults.   Rosano, Caterina  Phone:  
Email: rosanoc@edc.pitt.edu
Withdrawn  11/07/2013     
AS14-02  Predictors of the Change in Gait Speed with Exercise Training in the LIFE Pilot Study: A MicroRNA Analysis  Brinkley, Tina  Phone: (336) 713-8534  
Email: tbrinkle@wakehealth.edu
Withdrawn  11/13/2014  Pilot Study
 
We propose to examine microRNA (miRNA) expression profiles to identify potential biomarkers that distinguish individuals who improved their gait speed with the PA intervention from those who did not (i.e. responders vs. non-responders). We plan to use stored plasma samples and existing data that were collected as part of LIFE-P.  
AS11-05  Sleep Quality and Physical Activity in Older Adults   Endeshaw, Yohannes  Phone:  
Email: yendeshaw@aging.ufl.edu
Withdrawn  11/07/2013     
AS09-08  The LIFE Study: Changes in energy expenditure, physical function and fatigue   DeLaney, James D.  Phone: (412) 692-2297  
Email: jpd21@pitt.edu
Withdrawn  11/07/2013     
AS12-08  The LIFE Study: Muscle function and composition in the LIFE cohort: the impact of physical activity on muscle strength, power and skeletal muscle adiposity  Strotmeyer, Elsa  Phone: (412) 383-1293  
Email: strotmeyere@edc.pitt.edu
Withdrawn  12/22/2014    This ancillary will obtain a post-close out visit in order to determine if the physical activity intervention improved measure of muscle strength, power and skeletal muscle adiposity compared to the successful aging control group. 
AS12-04  The LIFE-COMBINE Study: Lifestyle Interventions and Independence for Elders - Cognition and Mobility Interface Study.  Nadkarni, Neelesh  Phone: (412) 692-2383  
Email: nadkarnink@upmc.edu
Withdrawn  01/06/2015    To examine relationships between dual-task costs (DTC) and physical performance (SPPB, 400m walk), and cognitive measures (3MS, DSST, Category Fluency, Trails A & B, HVLT, Modified Rey-O copy, MOCA and Boston naming test). 
AS09-06  The long-term effects of physical exercise on ectopic fat depots in the elderly: An ancillary study to LIFE Study  Ding, Jingzhong  Phone: (336) 713-8601  
Email: jding@wfubmc.edu
Withdrawn  11/07/2013     
AS12-15  The use of solid-phase extraction (SPE) of Aß from plasma to identify at risk individuals for cognitive decline   Felsenstein, Kevin  Phone: (352) 294-5308  
Email: kfelsenstein0@ufl.edu
Withdrawn  01/09/2015    This study will explore the Aß42:40 ratio determined from plasma after purification & concentration of the samples subsequent to solid-phase extraction (SPE). 
AS09-09  Training-Induced Bioenergetic Adaptations of Lower-Limb Muscles in Vivo in Older Adults  Kent-Braun, Jane  Phone: (413) 545-9477  
Email: janekb@kin.umass.edu
Withdrawn  11/07/2013     
AS09-04  Vascular Modulation in Mobility-Limited Elderly Following Exercise Training  Karas, Richard  Phone: (617) 636-8776  
Email: rkaras@tuftsmedicalcenter.org
Withdrawn  11/07/2013