LIFE Study Ancillary Tracking Summary Report

Approved and Conditionally Approved Ancillary Studies

Reference #	Title	Principle Investigator	Contact Information	Status*	Status Date	Completed/In Processs	Study Type	Summary of Ancillary Study
AS03-01	A lateral mobility task to assess mobility and transfer in older adults.	Marsh, Anthony	Phone: (336) 758-4643 Email: marshap@wfu.edu	Ancillary Study Approved	05/09/2013			We have developed a task to assess lateral mobility and transfer ability in older adults. It is a timed task that involves stepping over and ducking under obstacles and transferring into a chair. Therefore, the aim of this ancillary study is to further evaluate the construct validity and determine the predictive validity of the lateral mobility task and it’s sensitivity to change over time in the LIFE intervention and control groups.
AS10-02	A neuroimaging study to measure the association of baseline cerebral blood flow, structural brain characteristics and neuronal activation with the 2-year response to physical activity intervention in the LIFE –main participants.	Rosano, Caterina	Phone: Email: rosanoc@edc.pitt.edu	Ancillary Study Approved	04/05/2012			This proposal supplements resources already in place to test the hypothesis that baseline measures of brain integrity may be predictive of positive outcomes in response in physical activity. Additionally, we propose that PA changes are associated with differences in ultrastructure of hippocampal subregions and vascularization. We also propose to explore potential mechanisms underlying these associations, including levels of brain derived neurotrophic factors at the 2-year follow-up visit . Neuroimaging measures of brain integrity will include cutting–edge markers of cerebral blood flow and structural characteristics using a 7 Tesla magnet.
AS13-06	ACE pharmacogenetics and behavioral medicine to prevent age-related disability	Buford, Thomas	Phone: (352) 294-5800 Email: tbuford@ufl.edu	Ancillary Study Approved	07/25/2014			1) Determine if ACE I/D genotype influences the effect of ACEi+EX on changes in physical function among older adults. Based on our preliminary work, we hypothesize that changes in physical function in response to ACEi+EX will be poorest among persons with the II genotype. 2) Identify additional SNPs in ACE-related genes which may influence the effect of ACEi+EX. We propose to test the influence of other putative functional SNPs on changes in physical function in response to ACEi+EX. 3) Evaluate how the targeted genotypes influence changes in physical function in response to other antihypertensive medications (e.g. ARBs, diuretics) combined with exercise. These data will serve as a negative control to test that the hypothesized pharmacogenetic effects are specific to ACE inhibitor use. 4) Assess the influence of ACE-related genotypes on physical function in response to ACE inhibitor use and health education. These data will serve as a negative control to test our hypothesis that physical exercise is required to observe the hypothesized pharmacogenetic effects.
AS12-10	Analysis of Coding Variants Associated with Age-Related Phenotypes	Bowden, Donald	Phone: (336) 713-7507 Email: dbowden@wfubmc.edu	Ancillary Study Approved	03/22/2013			The aim of the proposed project is to examine common and uncommon coding variants for association with a wide range of biomedical measures characteristic of aging.
AS13-03	Autonomic function and gait variability in older adults at risk for mobility disability	Marsh, Anthony	Phone: (336) 758-4643 Email: marshap@wfu.edu	Ancillary Study Approved	09/13/2013			The aim of this ancillary study is to describe the degree of dysregulation in, and the relationship between, both autonomic function and gait using state-of-the-art non-linear dynamic analyses of HRV and step-to-step intervals of gait during both normal- and fast-paced walking. We will then examine whether dysfunction in HR and gait variability, either individually or in combination, is related to mobility. In addition, we will examine whether dysregulation in either HR or gait variability assessed during walking is moderated by confidence in balance, demographic characteristics (age, sex), and disease burden.
AS12-11	Cerebral metabolic, vascular and functional neuroimaging, NIH Toolbox cognitive measures and physical activity as predictors of age-associated cognitive dysfunction.	Cohen, Ron	Phone: (352) 294-5800 Email: roncohen@ufl.edu	Ancillary Study Approved	12/12/2012			Using previously established neuroimaging methods (T1 MPRAGE, FLAIR, DTI, MRS, ASL, resting BOLD), we propose to collect in vivo MRI measures from participants in the University of Florida cohort during a one hour multimodal scanning session. Data from this assessment will provide information regarding brain structural, metabolic/physiological and functional disturbances. Neurocognitive function will be examined relative to each neuroimaging modality using statistical modeling methods to determine the extent to which they predicts performance in specific cognitive domains (learning-memory, attention-executive, motor-processing speed). Coupling MRS, ASL and FMRI measures will enable us to examine how cerebral metabolic, vascular and functional changes are inter-related. Serum blood samples will also be collected and stored for analysis at a later date. Potential analyses include previously investigated serum biomarkers, such as IL-6, MCP-1, TNF- a, and BDNF.
AS13-07	CKD progression in older adults: the impact of geriatric conditions and physical activity	Liu, Christine	Phone: Email: christine.liu@bmc.org	Ancillary Study Approved	02/05/2014			Specific Aim 1: To determine if geriatric conditions are associated with increased risk of CKD progression in older adults with CKD. Hypothesis: Those who have exhaustion, weight loss, dizziness, depression, cognitive impairment, poor physical function, frailty, urinary problems, and/or falls at baseline will be more likely to develop CKD progression at 12 and 24 months. Specific Aim 2: To determine if physical activity can prevent CKD progression in older adults with CKD. Hypothesis: Those who are randomized to the physical activity (PA) group will be less likely to develop CKD progression at 12 and 24 months, compared to those randomized to the successful aging (SA) group. Exploratory Aim: To determine if inflammation is reduced after a program of physical activity in older adults with CKD. Hypothesis: In older adults with CKD, the inflammatory markers of interleukin-6 and C reactive protein will be reduced at 12 and 24 months in those randomized to the physical activity intervention, compared to those in successful aging.
AS09-10	Complex Mobility and Executive Functioning	Katula, Jeffrey	Phone: (336) 758-3612 Email: katulaj@wfu.edu	Ancillary Study Approved	02/22/2010			Participants will be required to complete 3 clinic visits in addition to the LIFE Study required visits (baseline, 12 mo, 24 mo). Each visit will take approximately 30 minutes. During each visit participants will complete 1 computerized cognitive assessment (10 min), the Walking Decision Making Task (8 min), and a complex walking task (from the INCHIANTI toolbox; 5 min).
AS12-06	Effects of a one-year physical activity program (LIFE-P) on serum C-terminal Agrin Fragment (CAF) concentrations among older adults at risk for physical disability	Buford, Thomas	Phone: (352) 294-5800 Email: tbuford@ufl.edu	Ancillary Study Approved	11/13/2012			Evaluate the effects of the LIFE-P interventions on circulating concentrations of a C-terminal Agrin Fragment (CAF)
AS03-03	Effects of exercise body composition in the elderly: the LIFE Study (DXA Body Comp)	Goodpaster, Bret	Phone: (412) 692-2437 Email: Bret.Goodpaster@FLHosp.org	Ancillary Study Approved	05/09/2013			This ancillary study proposes to obtain preliminary data in the LIFE study that will help address whether alterations in body composition, specifically changes in fat mass and lean mass with increased physical activity, predict reduced rates of incident mobility limitations. For this pilot phase of the LIFE study, we will determine whether loss of lean mass is similar to that observed older adults in Health ABC and determine whether estimate the effect size for determining whether the intervention attenuates the loss of lean mass
AS03-05	Effects of exercise on muscle strength and quality in elderly: The LIFE Study	Goodpaster, Bret	Phone: (412) 692-2437 Email: Bret.Goodpaster@FLHosp.org	Ancillary Study Approved	07/05/2005			This ancillary study proposes to obtain preliminary data in the LIFE study that will help address whether improvements in muscle quality with increased physical activity will predict reduced rates of incident mobility limitations in older subjects and that these improvements will be independent of changes in muscle mass or muscle strength.
AS05-01	Effects of exercise training on prevalence of metabolic syndrome in the elderly	Nicklas, Barbara	Phone: (336) 713-8037 Email: bnicklas@wfubmc.edu	Ancillary Study Approved	05/25/2005
AS04-01	Exercise and inflammatory risk factors for disability	Nicklas, Barbara	Phone: (336) 713-8037 Email: bnicklas@wfubmc.edu	Ancillary Study Approved	09/27/2004
AS05-03	Exercise, Brain Derived Neurotrophic Factor (BDNF) and Cognitive Decline	Garavoy, Natara	Phone: (650) 725-4420 Email: ngarovoy@stanford.edu	Ancillary Study Approved	10/27/2005			This study proposes to analyze existing serum samples from LIFE participants who completed assessments for cognitive decline at baseline and 12 month follow-up at the Stanford and Wake Forest sites. No additional data collection or analysis efforts will be requested from the participants or field centers. Data analyses will be conducted at Stanford.
AS12-14	Genetic associations with exercise-derived improvements in physical function among pre-disabled older adults: LIFE Genetics.	Buford, Thomas	Phone: (352) 294-5800 Email: tbuford@ufl.edu	Ancillary Study Approved	04/11/2013			we aim to conduct a genome-wide association study (GWAS) using DNA samples collected from participants in LIFE-M and LIFE-P
AS07-02	Genetic Polymorphisms in the Renin-Angiotensin System and Changes in Physical Function with Exercise Training	Ellis, Tina	Phone: (336) 713-8534 Email: tbrinkle@wfubmc.edu	Ancillary Study Approved	06/26/2007			This ancillary study will utilize existing DNA from all LIFE-P participants (regardless of age, gender or intervention assignment) who provided informed consent for DNA testing. We propose to generate biological data from the DNA specimens. Specifically, we will genotype DNA for polymorphisms in genes encoding components of the renin-angiotensin system (RAS), such as the angiotensin-converting enzyme (ACE), angiotensinogen (AGT), angiotensin II type 1 receptor (AT1R), bradykinin ß2 receptor (BKR2), and renin (REN) genes.
AS12-02	Impact of moderate physical activity on the longitudinal trajectory of cardiac specific biomarkers of stress and injury: support for modifying early heart failure phenotypes	deFilippi, Christopher	Phone: (410) 328-7204 Email: cdefilip@medicine.umaryland.edu	Ancillary Study Approved	09/25/2012			We propose to measure NT-proBNP,cTnT and cTnI in the Lifestyle Interventions and Independence for Elders (LIFE) pilot study and identifying if the frequency and extent of a rise in concentrations of any or all biomarkers are attenuated in those undergoing an active intervention of physical activity.
AS03-02	Is the age-associated decline in cardiorespiratory capacity attenuable/reversible through exercise training?	Church, Timothy	Phone: (225) 763-2632 Email: tim.church@pbrc.edu	Ancillary Study Approved	03/17/2004			The primary aim of this study is to assess and quantify the maximal cardiorespiratory capacity and the relative efficiency associated with exercise testing and those parameters influenced through exercise training. It is conceivable that little or no change in maximal exercise capacity may be observed; however, it is conceivable that sub-maximal exercise efficiency may improve and the quality of life of participating individuals will be related to these changes.
AS09-02	LIFE-KIDNEY Ancillary Study	Shlipak, Michael	Phone: (415) 221-4810 x3381 Email: michael.shlipak@ucsf.edu	Ancillary Study Approved	04/11/2011			The LIFE-KIDNEY ancillary study would extend the breadth of the trial by adding the kidney endpoints of kidney function (estimated glomerular filtration rate [GFR] from cystatin C and creatinine) and kidney damage (albuminuria). Both eGFR using cystatin C and albuminuria are very powerful prognostic markers in elderly persons, but no clinical trial in the elderly has ever attempted to delay the onset and progression of kidney disease. In the Cardiovascular Health Study (CHS), an observational study, physical inactivity was the strongest of all risk factors for more rapid decline in kidney function. Therefore, we believe that kidney endpoints would greatly enrich LIFE.
AS13-05	Metabolomic Predictors of Lean Mass and Physical Function in Functionally- Limited Older Adults	Fielding, Roger	Phone: (617) 556-3016 Email: roger.fielding@tufts.edu	Ancillary Study Approved	04/30/2014			Our overarching hypothesis is that specific serum metabolomic signatures can be identified that are: a) associated with lean mass and physical functioning, b) predictive of changes in lean mass and physical functioning in response to a physical activity intervention, and c) are altered in response to a physical activity intervention resulting from the underlying metabolic changes that result
AS11-02	Modification of HDL Function by Physical Training in the Elderly	Tang, W. H. Wilson	Phone: Email: tangw@ccf.org	Ancillary Study Approved	05/20/2014			The proposed studies will provide a comprehensive evaluation of the role that multiple pathways have in modifying HDL function, the development of CAD, and its major adverse complications such as coronary artery disease, heart failure, and death, as well as the relation between physical activity and these novel cardiometabolic risk factors by leveraging an ongoing physical activity intervention trial in the elderly population. Successful completion of the proposed studies will provide detailed mechanistic and clinical insights into the potential for novel cardiometabolic risks associated with modified HDL function to serve as a therapeutic target and/or diagnostic tools for cardiovascular diseases in at-risk patients.
AS12-13	mtDNA modifiers of the effect of exercise on cardiopulmonary and walking function in elders	Manini, Todd	Phone: (352) 294-5800 Email: tmanini@ufl.edu	Ancillary Study Approved	02/28/2013			Our central hypothesis is that mtDNA sequence variation explains a portion of the heterogeneity in cardiopulmonary and walking speed responses to regular PA
AS13-02	Novel biomarkers for response of muscle to exercise in older adults	McLean, Robert	Phone: (617) 971-5376 Email: rmclean@hsl.harvard.edu	Ancillary Study Approved	07/16/2013			The objective of this R01 proposal is to determine circulating biomarkers that are modulated by long-term exercise in older adults, and how they are associated with changes in muscle function. Our central hypothesis is that exercise decreases biomarkers of inflammatory cytokines and TGF-ß muscle atrophy signaling, and increases biomarkers of IGF-1 muscle hypertrophy signaling, and that these changes are associated with improvement in muscle function.
AS12-01	One-carbon metabolism and its relationship to physical and cognitive performance	Quinlivan, Eoin	Phone: (352) 273-5721 Email: epq@ufl.edu	Ancillary Study Approved	07/18/2012			This study will explore the relationship between one-carbon metabolism and physical (SPPB, 400 m walk, grip strength) and cognitive (DSST, Modified Stoop test, 3MSE, RAVLT) function measures, and the effect exercise has on this relationship
AS13-04	Peripheral metabolites associated with cognitive performance in participants of the Lifestyle Interventions and Independence for Elders Pilot study	Herzog, Raimund	Phone: Email: raimund.herzog@yale.edu	Ancillary Study Approved	07/10/2013			This study will explore the relationship between circulating peripheral plasma metabolites and cognitive function (as measured via a cognitive testing battery that includes the Modified Mini-Mental State Exam (3MSE); the Rey Auditory Verbal Learning Test (RAVLT) a test of working memory; the Digit Symbol Substitution Test (DSST), which tests mental speed and working memory and the Modified Stroop Test, which tests executive function). Based on compelling evidence from the literature, our focus will especially be on short chain acyl-carnitine species, which if deficient, have been associated with both physical and mental fatigue and decline
AS11-07	Physical Activity and Depressive Symptoms in LIFE-P: Effects of Genetic Polymorphisms and Symptom Dimensions of Depression	Dotson, Vonetta	Phone: Email: vonetta@phhp.ufl.edu	Ancillary Study Approved	02/14/2012			the goal of the proposed research is to help clarify the relationship between physical activity and depressive symptoms by examining 1) the effect of exercise on particular symptom dimensions of depression, 2) whether certain genetic markers moderate the effect of exercise on depressive symptoms, and 3) the relationships between depressive symptoms, exercise and cognitive functioning.
AS04-03	Physical Activity Monitoring In the LIFE Study Using the Actigraph Accelerometer	Haskell, William	Phone: (650) 725-5012 Email: whaskell@stanford.edu	Ancillary Study Approved	05/09/2013			Determine the capability of the Actigraph accelerometer activity monitor to detect a significant difference in the physical activity level in a subset of subjects assigned to the LIFE exercise group versus the healthy aging class at six months into the study. The two primary measures will be total activity per day over seven consecutive days as determined by (a) average number of “accelerometer counts” per hour and (b) number of minutes per day spent above a “conditioning threshold”.
AS11-08	Quantifying Physical Activity in the Physical Activity Intervention Using Accelerometry	Rejeski, Jack	Phone: (336) 758-5837 Email: rejeski@wfu.edu	Ancillary Study Approved	04/05/2012			Our interest is to evaluate the intensity and duration of walking activity, and to determine the number of bouts during a typical walking session (the number of breaks). These data will (a) help us to better understand how to interpret accelerometry data that is collected on similar populations in free-living environments, (b) provide a quantitative description of actual walking behavior that is being performed by participants in the LIFE study, and (c) enable us to determine whether there are reliable predictors of physical activity behavior in this context.
AS14-01	Role of Genetic Variation in the Impact of Physical Activity on Depressive Symptoms and Cognitive Functioning	Dotson, Vonetta	Phone: Email: vonetta@phhp.ufl.edu	Ancillary Study Approved	09/26/2014
AS06-02	Role of Vitamin D Status and VDR Polymorphisms on Physical Function	Houston, Denise	Phone: Email: dhouston@wakehealth.edu	Ancillary Study Approved	08/15/2006			To evaluate the role of vitamin D status (25(OH)D and PTH) and VDR polymorphisms on physical function in the LIFE Pilot Study, we propose to measure serum 25(OH)D, plasma PTH, and VDR polymorphisms at baseline. Vitamin D status was chosen because of the high prevalence of deficiency among the elderly and previously reported associations with physical function. There is a growing body of evidence that suggests that vitamin D status may be important in biologic processes that are important to the maintenance of physical function. There are also data to suggest that VDR polymorphisms may be associated with muscle strength and physical function.
AS05-04	Testosterone Level, SPPB Scores, and Symptoms among Older Men with Functional Limitations	Gill, Thomas	Phone: (203) 688-3344 Email: thomas.gill@yale.edu	Ancillary Study Approved	11/21/2005			This ancillary study will utilize existing blood specimens at each assessment time point from only the male LIFE participants who provided informed consent for phlebotomy. There is no extra time or effort required by participants
AS03-06	The effect of physical activity on vascular function in older adults (Pulse Waive Velocity)	Sutton-Tyrell, Kim	Phone: Email:	Ancillary Study Approved	04/13/2013			Among older adults enrolled in LIFE, a physical activity intervention will acutely reduce vascular stiffness. This may be accompanied by changes in CCA adventitial diameter. The study would involve baseline carotid scan and baseline and 6 month follow-up measures of aPWV and CCA adventitial diameter for about 50 participants at the Pittsburgh site only. If resources are available, we would like to do a third measure of aPWV and CCA adventitial diameter at 1 year.
AS10-01	The Effect of the Life Interventions on Global Gene Expression	Chupp, Geoffrey	Phone: Email: geoffrey.chupp@yale.edu	Ancillary Study Approved	09/18/2010
AS10-01	The Effect of the Life Interventions on Global Gene Expression	Chupp, Geoffrey	Phone: Email: geoffrey.chupp@yale.edu	Ancillary Study Approved	09/18/2010			This study will elucidate how the LIFE intervention affects global gene expression in the blood and will help to identify gene profiles of responsiveness to the LIFE intervention
AS11-06	The Effects of Exercise on Procollagen-3 N-Terminal Peptide (P3NP)	Pahor, Marco	Phone: (352) 294-5800 Email: mpahor@ufl.edu	Ancillary Study Approved	01/18/2012			This study will explore the effects of exercise on P3NP as well as the relationship between P3NP and physical performance measures (SPPB, 400 m walk), grip strength, IL-6, CRP, and DEXA measures of lean appendicular mass. The proposed ancillary study will utilize existing serum or plasma samples collected as a part of the LIFE Pilot study.
AS12-12	The impact of chronic kidney disease on the effectiveness of physical activity in older adults	Liu, Christine	Phone: Email: christine.liu@bmc.org	Ancillary Study Approved	11/28/2012			The data generated by this analysis will provide information on whether a physical activity to improve physical function is a feasible and effective intervention for older adults with CKD.
AS11-03	The LIFE Study: Mitochondrial Function and Exercise: Relationship with Function and Fatigue.	Goodpaster, Bret	Phone: (412) 692-2437 Email: Bret.Goodpaster@FLHosp.org	Ancillary Study Approved	06/09/2011			In this proposed pilot ancillary study, mitochondrial function will be measured directly and in-vivo via the novel and non-invasive method of 31P magnetic resonance spectroscopy (MRS). This new technology involves little participant burden, whereas the traditional method of muscle needle biopsy is highly invasive, induces considerable participant burden and must be performed by a physician.

Withdrawn Ancillary Studies

Previously approved studies that have been withdrawn are listed below. It is recommended that new proposers of ancillary studies contact the principal investigators of these withdrawn proposals, who may have some helpful insight for proposers of new ancillary studies.

Reference #	Title	Principle Investigator	Contact Information	Status*	Status Date	Completed/In Processs	Study Type	Summary of Ancillary Study
	Autonomic Function and Gait Variability in Older Adults At Risk for Mobility Disability	Marsh, Anthony	Phone: (336) 758-4643 Email: marshap@wfu.edu	Withdrawn	11/07/2013
AS12-09	Cerebral metabolic, vascular and functional neuroimaging and physical activity as predictors of age-associated cognitive dysfunction	Cohen, Ron	Phone: (352) 294-5800 Email: roncohen@ufl.edu	Withdrawn	06/12/2013
AS12-07	Effects of an Exercise Intervention on Vascular and Endothelial Function in the Elderly	Anton, Stephen	Phone: (352) 294-5800 Email: santon@ufl.edu	Withdrawn	01/06/2015			To test the dose-response effects of an exercise intervention on emerging CVD risk factors
AS06-01	Environmental Influences on Physical Activity in Older Adults at Heightened Risk for Disability	King, Abby	Phone: (650) 725-5394 Email: king@stanford.edu	Withdrawn	05/10/2013
AS03-04	Exercise and changes in Heart Rate Variability (HRV), in sedentary elderly men and women	Church, Timothy	Phone: (225) 763-2632 Email: tim.church@pbrc.edu	Withdrawn	05/09/2013
	Exploring the association of low levels of physical activity with disability in older adults	White, Dan	Phone: Email: dwtbn@bu.edu	Withdrawn	11/07/2013
	Is the age-associated decline in cardiorespiratory capacity attenuable/ reversible through exercise training (Fitness Testing)	Church, Timothy	Phone: (225) 763-2632 Email: tim.church@pbrc.edu	Withdrawn	11/07/2013
AS11-04	LIFE Study: The effect of physical activity on sleep-wake disturbances	Fragoso, Carlos	Phone: (203) 932-5711 Email: carlos.fragoso@yale.edu	Withdrawn	01/06/2015			The ancillary sleep testing that we propose to initiate at the 30 month follow-up visit (F30) is conducted only after the main study measures are completed. Specifically, after completing the F30 evaluation, LIFE staff will explain the ancillary sleep study to eligible participants. If enrolled, participants are consented and instructed on the sleep testing protocol. The actual sleep testing is then self-administered at the participant’s home, over 10-days. This includes an evaluation of sleep-wake disorders, namely circadian rhythm disorders, sleep apnea, and movement disorders
AS10-03	Machine learning of brain shape and composition for clinical prognosis in neural disorders	Laurienti, Paul	Phone: Email: plaurien@wfubmc.edu	Withdrawn	11/07/2013
AS09-01	Mitochondrial DNA and p53 damage in blood cells of the elderly: Correlation with physical activity (PA)	Sharp, J.G.	Phone: (402) 559-4390 Email: jsharp@unmc.edu	Withdrawn	05/10/2013
AS09-07	Muscle quality in the LIFE cohort: Impact of physical activity on changes in muscle strength, power, and composition in community-dwelling older adults at risk for mobility disability.	Goodpaster, Bret	Phone: (412) 692-2437 Email: Bret.Goodpaster@FLHosp.org	Withdrawn	11/07/2013
AS04-02	Napping and Sleep Practices of Older Adults: Relationship to Sleep Duration and Quality	Miller, Jennifer	Phone: (412) 383-1871 Email: miller.jennifer@medstudent.pitt.edu	Withdrawn	11/07/2013
AS09-11	NITRIC OXIDE Ancillary Study	Kim-Shapiro, Daniel	Phone: (336) 758-4993 Email: shapiro@wfu.edu	Withdrawn	11/07/2013
AS09-05	Non-exercise activity and risk for major mobility disability among older adults	Manini, Todd	Phone: (352) 294-5800 Email: tmanini@ufl.edu	Withdrawn	11/07/2013
AS05-02	Novel pathways of the disabling process and physical exercise	Cesari, Matteo	Phone: Email: macesari@gmail.com	Withdrawn	11/07/2013
	One-carbon metabolism and its relationship to physical and cognitive performance.	Quinlivan, Eoin	Phone: (352) 273-5721 Email: epq@ufl.edu	Withdrawn	11/07/2013
AS11-01	Physical activity and cardiovascular disease risk in older adults.	Manini, Todd	Phone: (352) 294-5800 Email: tmanini@ufl.edu	Withdrawn	11/07/2013
AS09-03	Physiological brain changes with increases in moderate physical activity among previously sedentary older adults.	Rosano, Caterina	Phone: Email: rosanoc@edc.pitt.edu	Withdrawn	11/07/2013
AS14-02	Predictors of the Change in Gait Speed with Exercise Training in the LIFE Pilot Study: A MicroRNA Analysis	Brinkley, Tina	Phone: (336) 713-8534 Email: tbrinkle@wfubmc.edu	Withdrawn	11/13/2014		Pilot Study	We propose to examine microRNA (miRNA) expression profiles to identify potential biomarkers that distinguish individuals who improved their gait speed with the PA intervention from those who did not (i.e. responders vs. non-responders). We plan to use stored plasma samples and existing data that were collected as part of LIFE-P.
AS11-05	Sleep Quality and Physical Activity in Older Adults	Endeshaw, Yohannes	Phone: Email: yendeshaw@aging.ufl.edu	Withdrawn	11/07/2013
AS09-08	The LIFE Study: Changes in energy expenditure, physical function and fatigue	DeLaney, James D.	Phone: (412) 692-2297 Email: jpd21@pitt.edu	Withdrawn	11/07/2013
AS12-08	The LIFE Study: Muscle function and composition in the LIFE cohort: the impact of physical activity on muscle strength, power and skeletal muscle adiposity	Strotmeyer, Elsa	Phone: (412) 383-1293 Email: strotmeyere@edc.pitt.edu	Withdrawn	12/22/2014			This ancillary will obtain a post-close out visit in order to determine if the physical activity intervention improved measure of muscle strength, power and skeletal muscle adiposity compared to the successful aging control group.
AS09-06	The long-term effects of physical exercise on ectopic fat depots in the elderly: An ancillary study to LIFE Study	Ding, Jingzhong	Phone: (336) 713-8601 Email: jding@wfubmc.edu	Withdrawn	11/07/2013
AS12-15	The use of solid-phase extraction (SPE) of Aß from plasma to identify at risk individuals for cognitive decline	Felsenstein, Kevin	Phone: (352) 294-5308 Email: kfelsenstein0@ufl.edu	Withdrawn	01/09/2015			This study will explore the Aß42:40 ratio determined from plasma after purification & concentration of the samples subsequent to solid-phase extraction (SPE).
AS09-09	Training-Induced Bioenergetic Adaptations of Lower-Limb Muscles in Vivo in Older Adults	Kent-Braun, Jane	Phone: (413) 545-9477 Email: janekb@kin.umass.edu	Withdrawn	11/07/2013
AS09-04	Vascular Modulation in Mobility-Limited Elderly Following Exercise Training	Karas, Richard	Phone: (617) 636-8776 Email: rkaras@tuftsmedicalcenter.org	Withdrawn	11/07/2013